Yes, no issues. I did this when I bought mine. I activated it on my laptop and when I got home from my business trip haven't used it since.
One thing to remember though...if you are SELLING the laptop or it's not yours, DEAUTHORIZE it from your iTunes account before you give it back.
I didn't do that, and there is nothing I can do until I fill up all the slots (you can authorize five computers). You can only deauthorize a computer that is connected to the iTunes OR deauthorize all five at once, once per year - BUT the option only comes up once you've filled the slots.