Some of us americans have been exposed to folks from other parts of the world, believe it or not. I learned this use of the word "queue" many years ago, though I would not use it to refer to people waiting in a line.(I am american, after all)...but I say stuff like "queue up" or "queless".
Lol.
Yes, many Americans probably know the term and its use, but we don't usually use it as a verb, because we tend to say "line up." Mostly, I hear it used as a noun in America, like when someone says something is in a queue (sometimes as an online term, too).
You also tend to hear it used more often as a verb among East Coasters, or others who are well traveled or well read, exposed to culture beyond that of the U.S. (many of us, fortunately, lol).
Speaking of Britishisms, this might be of interest:
America?s Slippery Slope Into Britishisms - NYTimes.com